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KMID : 1098420090170020145
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 2 p.145 ~ p.150
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Magnolol Attenuates Neuronal Cell Death Induced by Kynurenine Metabolite
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Lee Chang-Uk
Lee Hyun-Jung
Kim Do-Hee
Jang Yeong-Mi
Lee Sang-Hyung
Jeong Yoon-Hwa
Kim Dae-Jin
Kim Kyung-Yong
Kim Sung-Su
Lee Won-Bok
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Abstract
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This study investigated the protective roles and mechanism of magnolol, from the stem bark of Magnolia officinalis against potential neurotoxin 3-hydroxykynurenine (3-HK)-induced neuronal cell death. For the evaluation of protective role of magnolol, we examined cell viability, apoptotic nuclei, change of mitochondrial membrane potential and caspase activity in human neuroblastoma SH-SY5Y cells. It was found that 3-HK induces neuronal cell death in the human neuroblastoma SH-SY5Y cell line. The reduced cell viability produced characteristic features such as cell shrinkages, plasma membrane blebbing, chromatin condensation, and nuclear fragmentation. The cells treated with 3-HK showed an increase in the concentration of reactive oxygen species (ROS) as well as in caspase activity. In addition, both are involved in the 3-HK-induced apoptosis. Magnolol attenuated the cell viability reduction by 3-HK in both a dose- and time-dependent manner. Optical microscopy showed that magnolol inhibited the cell morphological features in the 3-HK-treated cells. Furthermore, the increase in the ROS concentration and the caspase activities by 3-HK were also attenuated by magnolol. These results showed that magnolol has a protective effect on the 3-HK induced cell death by inhibiting ROS production and caspase activity.
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KEYWORD
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Magnolol, 3-hydroxykynureinine, Mitochondria, ROS, Caspase-3
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